1994-1998 Peking University-B.S. in Cell Biology and Genetics
1998-2003 Peking University-Ph.D. in Cell Biology and Genetics

2004-2010 Post-doctoral Research Associate, University of Cambridge
2010-Present Principal Investigator, BIOPIC, College of Life Sciences, Peking University
2017-Present Professor, BIOPIC, College of Life Sciences, Peking University
2015-Present Member of Peking-Tsinghua Center for Life Sciences

Top 10 Scientific and Technological Progresses of China in the year of 2014
Top 10 Scientific and Technological Progresses of China in the year of 2015
Top 10 Scientific Advances in Life Sciences of China in the year of 2015
Top 10 Scientific Advances in Life Sciences of China in the year of 2019

2014 - 2017 Science Bulletin, Editorial Board Member
2015 - Present Genome Biology, Editorial Board Member
2016 - Present Open Biology, Editorial Board Member
2020 - Present Cell Stem Cell, Editorial Board Member

Stem cells have self-renewal ability and multi-lineage differentiation potential and are crucial for human embryonic development and adult life. Dissecting the molecular mechanisms of stem cell regulation is important for understanding human growth and development, and for its application of regeneration medicine for cure of human diseases. My lab focuses on the epigenetic regulation of human germline cell development as well as pluripotency and self-renewal ability of human pluripotent stem cells. Through this, we want to understand how the epigenetic factors regulate the gene expression network during human early embryonic development and germline development. We systematically developed single cell functional genomic sequencing technologies (single cell RNA-Seq, single cell DNA methylome sequencing, single cell COOL-seq, single cell CLEVER-seq, single cell Trio-seq techniques, etc.) and used these technologies as well as other cutting-edge technologies (gene editing, micromanipulation of mammalian embryos, culture of organoids, directed differentiations of human pluripotent stem cells, etc.) to decipher the molecular mechanisms of the gene regulation network in human early embryos and germline cells at single-cell and single-base resolution. Furthermore, we also explore the molecular mechanisms of germline cell disorders in human infertility situations.

1. Li L, Li L, Li Q, Liu X, Ma X, Yong J, Gao S, Wu X, Wei Y, Wang X, Wang W, Li R, Yan J, Zhu X, Wen L, Qiao J, Yan L*, Tang Fuchou*. Dissecting the epigenomic dynamics of human fetal germ cell development at single-cell resolution. Cell Research, doi.org/10.1038/s41422-020-00401-9 (2020) (*: Co-corresponding authors).
2. Fan X, Fu Y, Zhou X, Sun L, Yang M, Wang M, Chen R, Wu Q, Yong J, Dong J, Wen L, Qiao J*, Wang X*, Tang Fuchou*. Single-cell transcriptome analysis reveals cell lineage specification in temporal-spatial patterns in human cortical development. Science Advances, 6: eaaz2978 (2020) (*: Co-corresponding authors).
3. Gao Y, Li L, Yuan P, Zhai F, Ren Y, Yan L, Li R, Lian Y, Zhu X, Wu X, Kee K, Wen L, Qiao J*, Tang Fuchou*. 5-Formylcytosine landscapes of human preimplantation embryos at single-cell resolution. PLOS Biology, doi.org/10.1371/journal.pbio.3000799 (2020) (*: Co-corresponding authors).
4. Yu K, Hu Y, Wu F, Guo Q, Qian Z, Hu W, Chen J, Wang K, Fan X, Wu X, Rasko J, Fan X, Iavarone A*, Jiang T*, Tang Fuchou*, Su X*. Surveying brain tumor heterogeneity by single-cell RNA sequencing of multi-sector biopsies. National Science Review, doi.org/10.1093/nsr/nwaa099 (2020) (*: Co-corresponding authors).
5. Li J, Zheng Y, Yan P, Song M, Wang S, Sun L, Liu Z, Ma S, Izpisua Belmonte JC, Chan P, Zhou Q, Zhang W*, Liu G*, Tang Fuchou*, Qu J*. A Single-cell Transcriptomic Atlas of Primate Pancreatic Islet Aging. National Science Review, doi.org/10.1093/nsr/nwaa127 (2020) (*: Co-corresponding authors).
6. Zhang W, Zhang S, Yan P, Ren J, Song M, Li J, Lei J, Pan H, Wang S, Ma X, Ma S, Li H, Sun F, Wan H, Li W, Chan P, Zhou Q, Liu GH*, Tang Fuchou*, Qu J*. A single-cell transcriptomic landscape of primate arterial aging. Nature Communications, 11: 2202 (2020) (*: Co-corresponding authors).
7. Hou S, Li Z, Zheng X, Gao Y, Dong J, Ni Y, Wang X, Li Y, Ding X, Chang Z, Li S, Hu Y, Fan X, Hou Y, Wen L, Liu B*, Tang Fuchou*, Lan Y*. Embryonic endothelial evolution towards first hematopoietic stem cells revealed by single-cell transcriptomic and functional analyses. Cell Research, 30: 376-392 (2020) (*: Co-corresponding authors).
8. Zhang XM, Wu K, Zheng Y, Zhao H, Gao J, Hou Z, Zhang M, Liao J, Zhang J, Gao Y, Li Y, Li L, Tang Fuchou*, Chen ZJ*, Li J*. In vitro expansion of human sperm through nuclear transfer. Cell Research, 30: 356-359 (2020) (*: Co-corresponding authors).
9. Chen Y, Lyu R, Rong B, Zheng Y, Lin Z, Dai R, Zhang X, Xie N, Wang S, Tang Fuchou*, Lan F*, Tong MH*. Refined spatial temporal epigenomic profiling reveals intrinsic connection between PRDM9-mediated H3K4me3 and the fate of double-stranded breaks. Cell Research, 30: 256-268 (2020) (*: Co-corresponding authors).
10. Wang S, Zheng Y, Li J, Yu Y, Zhang W, Song M, Liu Z, Min Z, Hu H, Jing Y, He X, Sun L, Ma L, Esteban CR, Chan P, Qiao J, Zhou Q, Izpisua Belmonte JC*, Qu J*, Tang Fuchou*, Liu GH*. Single-Cell Transcriptomic Atlas of Primate Ovarian Aging. Cell, 180: 585-600 (2020) (*: Co-corresponding authors).
11. Li J, Wang R, Zhou X, Wang W, Gao S, Mao Y, Wu X, Guo L, Liu H, Wen L, Fu W*, Tang Fuchou*. Genomic and transcriptomic profiling of carcinogenesis in patients with familial adenomatous polyposis. Gut, 69: 1283-1293 (2020) (*: Co-corresponding authors).
12. Wen L, Liu Q, Xu J, Liu X, Shi C, Yang Z, Zhang Y, Xu H, Liu J*, Yang H*, Huang H*, Qiao J*, Tang Fuchou*, Chen ZJ*. Recent advances in mammalian reproductive biology. Science China Life Sciences, 63: 18-58 (2020) (*: Co-corresponding authors).
13. Wen L*, Tang Fuchou*. Human Germline Cell Development: from the Perspective of Single-Cell Sequencing. Molecular Cell, 76: 320-328 (2019) (Review) (*: Co-corresponding authors).
14. Yang X, Hu B, Liao J, Qiao Y, Chen Y, Qian Y, Feng S, Yu F, Dong J, Hou Y, Xu H, Wang R, Peng G*, Li J*, Tang Fuchou*, Jing N*. Distinct enhancer signatures in the mouse gastrula delineate progressive cell fate continuum during embryo development. Cell Research, 29: 911-926 (2019) (*: Co-corresponding authors).
15. Zhou F, Wang R, Yuan P, Ren Y, Mao Y, Li R, Lian Y, Li J, Wen L, Yan L, Qiao J*, Tang Fuchou*. Reconstituting the transcriptome and DNA methylome landscapes of human implantation. Nature, 572: 660-664 (2019) (*: Co-corresponding authors).
16. Soares E, Xu Q, Li Q, Qu J, Zheng Y, Raeven HHM, Brandao KO, Petit I, van den Akker WMR, van Heeringen SJ, Aberdam D, Tang Fuchou*, Zhou H*. Single-cell RNA-seq identifies a reversible mesodermal activation in abnormally specified epithelia of p63 EEC syndrome. Proc Natl Acad Sci U S A, 116: 17361-17370 (2019) (*: Co-corresponding authors).
17. Hu Y, Wang X, Hu B, Mao Y, Chen Y, Yan L, Yong J, Dong J, Wei Y, Wang W, Wen L, Qiao J*, Tang Fuchou*. Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis. PLoS Biology, 17: e3000365 (2019) (*: Co-corresponding authors).
18. Fu L, Hu Y, Song M, Liu Z, Zhang W, Yu FX, Wu J, Wang S, Izpisua Belmonte JC, Chan P, Qu J*, Tang Fuchou*, Liu GH*. Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis. PLoS Biology, 17: e3000201 (2019) (*: Co-corresponding authors).
19. Ren X, Hu B, Song M, Ding Z, Dang Y, Liu Z, Zhang W, Ji Q, Ren R, Ding J, Chan P, Jiang C, Ye K, Qu J*, Tang Fuchou*, Liu GH*. Maintenance of Nucleolar Homeostasis by CBX4 Alleviates Senescence and Osteoarthritis. Cell Reports, 26: 3643-3656 (2019) (*: Co-corresponding authors).
20. Cui Y, Zheng Y, Liu X, Yan L, Fan X, Yong J, Hu Y, Dong J, Li Q, Wu X, Gao S, Li J, Wen L, Qiao J*, Tang Fuchou*. Single-cell transcriptome analysis maps the developmental track of the human heart. Cell Reports, 26: 1934–1950 (2019) (*: Co-corresponding authors).
21. Yan P, Li Q, Wang L, Lu P, Suzuki K, Liu Z, Lei J, Li W, He X, Wang S, Ding J, Chan P, Zhang W, Song M, Izpisua Belmonte JC, Qu J*, Tang Fuchou*, Liu GH*. FOXO3-engineered human ESC-derived vascular cells promote vascular protection and regeneration. Cell Stem Cell, 24: 447-461 (2019) (*: Co-corresponding authors).
22. Ji Q, Zheng Y, Zhang G, Hu Y, Fan X, Hou Y, Wen L, Li L, Xu Y, Wang Y*, Tang Fuchou*. Single-cell RNA-seq analysis reveals the progression of human osteoarthritis. Annals of the Rheumatic Diseases, 78: 100-110 (2019) (*: Co-corresponding authors).
23. Bian S, Hou Y, Zhou X, Li X, Yong J, Wang Y, Wang W, Yan J, Hu B, Guo H, Wang J, Gao S, Mao Y, Dong J, Zhu P, Xiu D, Yan L, Wen L, Qiao J*, Tang Fuchou*, Fu W*. Single-cell multiomics sequencing and analyses of human colorectal cancer. Science, 362: 1060-1063 (2018) (*: Co-corresponding authors).
24. Wang P, Chen Y, Yong J, Cui Y, Wang R, Wen L, Qiao J*, Tang Fuchou*. Dissecting the global dynamic molecular profiles of human fetal kidney development by single-cell RNA sequencing. Cell Reports, 24: 3554-3567 (2018) (*: Co-corresponding authors).
25. Wang M, Liu X, Chang G, Chen Y, An G, Yan L, Gao S, Xu Y, Cui Y, Dong J, Chen Y, Fan X, Hu Y, Song K, Zhu X, Gao Y, Yao Z, Bian S, Hou Y, Lu J, Wang R, Fan Y, Lian Y, Tang W, Wang Y, Liu J, Zhao L, Wang L, Liu Z, Yuan R, Shi Y, Hu B, Ren X, Tang Fuchou*, Zhao XY*, Qiao J*. Single-cell RNA sequencing analysis reveals sequential cell fate transition during human spermatogenesis. Cell Stem Cell, 23: 599-614 (2018) (*: Co-corresponding authors).
26. Chen Y, Zheng Y, Gao Y, Lin Z, Yang S, Wang T, Wang Q, Xie N, Hua R, Liu M, Sha J, Griswold MD, Li J*, Tang Fuchou*, Tong MH*. Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis. Cell Research, 28: 879–896 (2018) (Cover story) (*: Co-corresponding authors).
27. Li L, Guo F, Gao Y, Ren Y, Yuan P, Yan L, Li R, Liang Y, Li J, Hu B, Gao J, Wen L, Tang Fuchou*, Qiao J*. Single-cell multi-omics sequencing of human early embryos. Nature Cell Biology, 20: 847-858 (2018) (*: Co-corresponding authors).
28. Fan X, Dong J, Zhong S, Wei Y, Wu Q, Yan L, Yong J, Sun L, Wang X, Zhao Y, Wang W, Yan J, Wang X*, Qiao J*, Tang Fuchou*. Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis. Cell Research, 28: 730-745 (2018) (*: Co-corresponding authors).
29. Gao S, Yan L, Wang R, Li J, Yong J, Zhou X, Wei Y, Wu X, Wang X, Fan X, Yan J, Zhi X, Gao Y, Guo H, Jin X, Wang W, Mao Y, Wang F, Wen L, Fu W, Ge H*, Qiao J*, Tang Fuchou*. Tracing the temporal-spatial transcriptome landscapes of the human fetal digestive tract using single-cell RNA-sequencing. Nature Cell Biology, 20: 721-734 (2018) (*: Co-corresponding authors).
30. Zhong S, Zhang S, Fan X, Wu Q, Yan L, Dong J, Zhang H, Li L, Sun L, Pan N, Xu X, Tang Fuchou*, Zhang J*, Qiao J*, Wang X*. A single-cell RNA-seq survey of the developmental landscape of the human prefrontal cortex. Nature, 555: 524-528 (2018) (*: Co-corresponding authors).
31. Zhu P, Guo H, Ren Y, Hou Y, Dong J, Li R, Lian Y, Fan X, Hu B, Gao Y, Wang X, Wei Y, Liu P, Yan J, Ren X, Yuan P, Yuan Y, Yan Z, Wen L, Yan L*, Qiao J*, Tang Fuchou*. Single-cell DNA methylome sequencing of human preimplantation embryos. Nature Genetics, 50: 12-19 (2018) (*: Co-corresponding authors).
32. Wen L*, Tang Fuchou*. Boosting the power of single-cell analysis. Nature Biotechnology, 36: 408-409 (2018) (Preview) (*: Co-corresponding authors).
33. Dong J, Hu Y, Fan X, Wu X, Mao Y, Hu B, Guo H, Wen L, Tang Fuchou*. Single-cell RNA-seq analysis unveils a prevalent epithelial/mesenchymal hybrid state during mouse organogenesis. Genome Biology, 19: 31 (2018) (*: Corresponding author).
34. Yang X, Hu B, Hou Y, Qiao Y*, Wang R, Chen Y, Qian Y, Feng S, Chen J, Liu C, Peng G, Tang Fuchou*, Jing N*. Silencing of developmental genes by H3K27me3 and DNA methylation reflects the discrepant plasticity of embryonic and extraembryonic lineages. Cell Research, 28: 593-596 (2018) (*: Co-corresponding authors).
35. Wang S, Hu B, Ding Z, Dang Y, Wu J, Li D, Liu X, Xiao B, Zhang W, Ren R, Lei J, Hu H, Chen C, Chan P, Li D, Qu J*, Tang Fuchou*, Liu GH*. ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells. Cell Discovery, 4: 2 (2018) (*: Co-corresponding authors).
36. Wen L* & Tang Fuchou*. Single cell epigenome sequencing technologies. Molecular Aspects of Medicine, 59: 62-69 (2018) (Review) (*: Co-corresponding authors).
37. Liu J, Liu W, Yang L, Wu Q, Zhang H, Fang A, Li L, Xu X, Sun L, Zhang J*, Tang Fuchou*, Wang X*. The primate-specific gene TMEM14B marks outer radial glia cells and promotes cortical expansion and folding. Cell Stem Cell, 21: 635-649 (2017) (*: Co-corresponding authors).
38. Guo F*, Li L, Li J, Wu X, Hu B, Zhu P, Wen L, Tang Fuchou*. Single-cell multi-omics sequencing of mouse early embryos and embryonic stem cells. Cell Research, 27: 967-988 (2017) (Cover story) (*: Co-corresponding authors).
39. Yang J, Li J, Suzuki K, Liu X, Wu J, Zhang W, Ren R, Zhang W, Chan P, Izpisua Belmonte JC, Qu J*, Tang Fuchou*, Liu GH*. Genetic enhancement in cultured human adult stem cells conferred by a single nucleotide recoding. Cell Research, 27: 1178-1181 (2017) (*: Co-corresponding authors).
40. Li L, Dong J, Yan L, Yong J, Liu X, Hu Y, Fan X, Wu X, Guo H, Wang X, Zhu X, Li R, Yan J, Wei Y, Zhao Y, Wang W, Ren Y, Yuan P, Yan Z, Hu B, Guo F, Wen L, Tang Fuchou*, Qiao J*. Single-cell RNA-seq analysis maps development of human germline cells and gonadal niche interactions. Cell Stem Cell, 20: 858-873 (2017) (*: Co-corresponding authors).
41. Zhu C, Gao Y, Guo H, Xia B, Song J, Wu X, Zeng H, Kee K, Tang Fuchou*, Yi C*. Single-cell 5-formylcytosine landscapes of mammalian early embryos and ESCs at single-base resolution. Cell Stem Cell, 20: 720-731 (2017) (*: Co-corresponding authors).
42. Guo H, Hu B, Yan L, Yong J, Wu Y, Gao Y, Guo F, Hou Y, Fan X, Dong J, Wang X, Zhu X, Yan J, Wei Y, Jin H, Zhang W, Wen L, Tang Fuchou*, Qiao J*. DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells. Cell Research, 27: 165-183 (2017) (*: Co-corresponding authors).
43. Yang L, Ma Z, Cao C, Zhang Y, Wu X, Lee R, Hu B, Wen L, Ge H, Huang Y*, Lao K*, Tang Fuchou*. MR-seq: measuring a single cell’s transcriptome repeatedly by RNA-seq. Science Bulletin, 62: 391-398 (2017) (*: Co-corresponding authors).
44. Zhou F, Li X, Wang W, Zhu P, Zhou J, He W, Ding M, Xiong F, Zheng X, Li Z, Ni Y, Mu X, Wen L, Cheng T, Lan Y, Yuan W*, Tang Fuchou*, Liu B*. Tracing haematopoietic stem cell formation at single-cell resolution. Nature, 533: 487-492 (2016) (*: Co-corresponding authors).
45. Wen L*, Tang Fuchou*. Single-cell sequencing in stem cell biology. Genome Biology 17: 71 (2016) (Review) (*: Co-corresponding authors).
46. Dang Y, Yan L, Hu B, Fan X, Ren Y, Li R, Lian Y, Yan J, Li Q, Zhang Y, Li M, Ren X, Huang J, Wu Y, Liu P, Wen L, Zhang C, Huang Y*, Tang Fuchou*, Qiao J*. Tracing the expression of circular RNAs in human pre-implantation embryos. Genome Biology 17: 130 (2016) (*: Co-corresponding authors).
47. Hou Y, Guo H, Cao C, Li X, Hu B, Zhu P, Wu X, Wen L, Tang Fuchou*, Huang Y*, Peng J*. Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas. Cell Research 26: 304-319 (2016) (*: Co-corresponding authors).
48. Pan H, Guan D, Liu X, Li J, Wang L, Wu J, Zhou J, Zhang W, Ren R, Zhang W, Li Y, Yang J, Hao Y, Yuan T, Yuan G, Wang H, Ju Z, Mao Z, Li J, Qu J*, Tang Fuchou*, Liu GH*. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Research 26: 190-205 (2016) (*: Co-corresponding authors).
49. Yan L, Guo H, Hu B, Li R, Yong J, Zhao Y, Zhi X, Fan X, Guo F, Wang X, Wang W, Wei Y, Wang Y, Wen L, Qiao J*, Tang Fuchou*. Epigenomic landscape of human fetal brain, heart, and liver. Journal Biological Chemistry 291: 4386-4398 (2016) (*: Co-corresponding authors).
50. Yan L, Huang L, Xu L, Huang J, Ma F, Zhu X, Tang Y, Liu M, Lian Y, Liu P, Li R, Lu S, Tang Fuchou*, Qiao J*, Xie XS*. Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses. Proc Natl Acad Sci U S A 112: 15964-15969 (2015) (*: Co-corresponding authors).
51. Wen L*, Tang Fuchou*, How to catch rare cell types. Nature 52: 197-198 (2015) (*: Co-corresponding authors) (Preview).
52. Guo F, Yan L, Guo H, Li L, Hu B, Zhao Y, Yong J, Hu Y, Wang X, Wei Y, Wang W, Li R, Yan J, Zhi X, Zhang Y, Jin H, Zhang W, Hou Y, Zhu P, Li J, Zhang L, Liu S, Ren Y, Zhu X, Wen L, Gao Y, Tang Fuchou*, Qiao J*. The transcriptome and DNA methylome landscapes of human primordial germ cells. Cell 161: 1437-1452 (2015) (*: Co-corresponding authors).
53. Zhang W, Li J, Suzuki K, Qu J, Wang P, Zhou J, Liu X, Ren R, Xu X, Ocampo A, Yuan T, Yang J, Li Y, Shi L, Guan D, Pan H, Suan S, Ding Z, Li M, Yi F, Bai R, Wang Y, Chen C, Yang F, Li X, Wang Z, Aizawa E, Goebl A, Soligalla RE, Reddy P, Esteban CR, Tang Fuchou*, Liu G* and Izpisua Belmonte JC*. A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging. Science 348: 1160-1163 (2015) (*: Co-corresponding authors).
54. Wen L*, Tang Fuchou*. Charting a map through the cellular reprogramming landscape. Cell Stem Cell 16: 215-216 (2015) (*: Co-corresponding authors) (Preview).
55. Wen L, Li J, Guo H, Liu X, Zheng S, Zhang D, Zhu W, Qu J, Guo L, Du D, Jin X, Zhang Y, Gao Y, Shen J, Ge H, Tang Fuchou*, Huang Y*, Peng J*. Genome-scale detection of hypermethylated CpG islands in circulating cell-free DNA of hepatocellular carcinoma patients. Cell Research 25: 1250-1264 (2015) (*: Co-corresponding authors).
56. Duan S, Yuan G, Liu X, Ren R, Li J, Zhang W, Wu J, Xu X, Fu L, Li Y, Yang J, Zhang W, Bai R, Yi F, Suzuki K, Gao H, Esteban CR, Zhang C, Izpisua Belmonte JC, Chen Z, Wang X, Jiang T, Qu J*, Tang Fuchou*, Liu GH*. PTEN deficiency reprogrammes human neural stem cells towards a glioblastoma stem cell-like phenotype. Nature Communications 6: 10068 (2015) (*: Co-corresponding authors).
57. Fan X, Zhang X, Wu X, Guo H, Hu Y, Tang Fuchou*, Huang Y*. Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos. Genome Biology 16: 148 (2015) (*: Co-corresponding authors).
58. Guo H, Zhu P, Guo F, Li X, Wu X, Fan X, Wen L*, Tang Fuchou*. Profiling DNA methylome landscapes of mammalian cells with single-cell reduced-representation bisulfite sequencing. Nature Protocols 10: 645-659 (2015) (*: Co-corresponding authors).
59. Wu Y, Zhou H, Fan X, Zhang Y, Zhang M, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L* , Tang Fuchou*, Li J,* Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Cell Research 25: 67-79 (2015) (*: Co-corresponding authors).
60. Shen J, Jiang D, Fu Y, Wu X, Guo H, Feng B, Pang Y, Streets AM, Tang Fuchou*, Huang Y*. H3K4me3 epigenomic landscape derived from ChIP-Seq of 1 000 mouse early embryonic cells. Cell Research 25: 143-147 (2015) (*: Co-corresponding authors).
61. Guo F, Li X, Liang D, Li T, Zhu P, Guo H, Wu X, Wen L, Gu TP, Hu B, Walsh CP, Li J*, Tang Fuchou*, Xu GL*. Active and passive demethylation of male and female pronuclear dna in the mammalian zygote. Cell Stem Cell 15: 447-458 (2014) (*: Co-corresponding authors).
62. Guo H, Zhu P, Yan L, Li R, Hu B, Lian Y, Yan J, Ren X, Lin S, Li J, Jin X, Shi X, Liu P, Wang X, Wang W, Wei Y, Li X, Guo F, Wu X, Fan X, Yong J, Wen L, Xie SX, Tang Fuchou*, Qiao J*. The DNA methylation landscape of human early embryos. Nature 511: 606-610 (2014) (*: Co-corresponding authors).
63. Wen L, Tang Fuchou*. Reconstructing complex tissues from single-cell analyses. Cell 157: 771-773 (2014) (*: Corresponding author) (Preview).
64. Streets AM, Zhang X, Cao C, Pang Y, Wu X, Xiong L, Yang L, Fu Y, Zhao L*, Tang Fuchou*, Huang Y*. Microfluidic single-cell whole-transcriptome sequencing. Proc Natl Acad Sci U S A 111: 7048-7053 (2014) (*: Co-corresponding authors).
65. Wen L, Li X, Yan L, Tan Y, Li R, Zhao Y, Wang Y, Xie J, Zhang Y, Song C, Yu M, Liu X, Zhu P, Li X, Hou Y, Guo H, Wu X, He C*, Li R*, Tang Fuchou*, Qiao J*. Whole-genome analysis of 5-hydroxymethylcytosine and 5-methylcytosine at base resolution in the human brain. Genome Biology 15: R49 (2014) (*: Co-corresponding authors).
66. Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, Li J, Xu L, Tang Fuchou*, Xie XS*, Qiao J*. Genome analyses of single human oocytes. Cell 155:1492-1506 (2013) (*: Co-corresponding authors).
67. Guo H, Zhu P, Wu X, Li X, Wen L, Tang Fuchou*. Single-cell methylome landscapes of mouse embryonic stem cells and early embryos analyzed using reduced representation bisulfite sequencing. Genome Research 23: 2126-2135 (2013) (*: Corresponding author).
68. Yan L, Yang M, Guo H, Yang L, Wu J, Li R, Liu P, Lian Y, Zheng X, Yan J, Huang J, Li M, Wu X, Wen L, Lao K, Li R*, Qiao J*, Tang Fuchou*. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells. Nature Structural & Molecular Biology 20: 1131-1139 (2013) (*: Co-corresponding authors).
69. Gan H, Wen L, Liao S, Lin X, Ma T, Liu J, Song CX, Wang M, He C, Han C*, Tang Fuchou*. Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis. Nature Communications 4: 1995 (2013) (*: Co-corresponding authors).
70. Tang Fuchou*, Barbacioru C*, Nordman E, Bao S, Lee C, Wang X, Tuch BB, Heard E, Lao K, Surani MA. Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One 6: e21208 (2011) (*: Co-first authors).
71. Tang Fuchou, Lao K, Surani MA. Development and applications of single-cell transcriptome analysis. Nature Methods 8: S6-S11 (2011) (Review).
72. Tang Fuchou, Barbacioru C, Bao S, Lee C, Nordman E, Wang X, Lao K, Surani MA. Tracing the derivation of embryonic stem cells from the inner cell mass by single-cell RNA-Seq analysis. Cell Stem Cell 6: 468 - 478 (2010).
73. Tang Fuchou*. Small RNAs in mammalian germline: tiny for immortal. Differentiation 79: 141-146 (2010) (*: Corresponding author) (Review).
74. Tang Fuchou, Barbacioru C, Nordman E, Li B, Xu N, Bashkirov VI, Lao K, Surani MA. RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nature Protocols 5: 516-535 (2010).
75. Bao S*, Tang Fuchou*, Li X, Hayashi K, Gillich A, Lao K, Surani MA. Epigenetic reversion of postimplantation epiblast to pluripotent embryonic stem cells. Nature 461: 1292 - 1295 (2009) (*: Co-first authors).
76. Tang Fuchou*, Barbacioru C*, Wang Y, Nordman E, Lee C, Xu N, Wang X, Bodeau J, Tuch BB, Siddiqui A, Lao K, Surani MA. mRNA-Seq whole transcriptome analysis of a single cell. Nature Methods 6: 377 - 382 (2009) (*: Co-first authors).
77. Tang Fuchou, Hajkova P, O`Carroll D, Lee C, Tarakhovsky A, Lao K, Surani MA. MicroRNAs are tightly associated with RNA-induced gene silencing complexes in vivo. Biochemical & Biophysical Research Communications 372: 24 - 29 (2008).
78. Tang Fuchou, Hayashi K, Kaneda M, Lao K, Surani MA. A sensitive multiplex assay for piRNA expression. Biochemical & Biophysical Research Communications 369: 1190 - 1194 (2008).
79. Tang Fuchou*, Kaneda M*, O`Carroll D, Hajkova P, Barton SC, Sun YA, Lee C, Tarakhovsky A, Lao K, Surani MA. Maternal microRNAs are essential for mouse zygotic development. Genes & Development 21: 644 - 648 (2007) (*: Co-first authors).
80. Tang Fuchou, Hajkova P, Barton SC, O`Carroll D, Lee C, Lao K, Surani MA. 220-plex microRNA expression profile of a single cell. Nature Protocols 1: 1154 - 1159 (2006).
81. Tang Fuchou, Hajkova P, Barton SC, Lao K, Surani MA. MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Research 34: e9 (2006).